Some restriction enzymes create blunt DNA ends, without the complementary single-stranded overhangs of sticky ends. What is the advantage of using restriction enzymes that create sticky ends vs. blunt ends for creating recombinant
DNA molecules? What will be an ideal response?
Since sticky ends are complementary, they will pair up easily, forming hydrogen bonds
between base pairs. This allows DNA ligase to create covalent bonds between each DNA
fragment. Blunt ends do not have complementary ends and therefore do not pair up easily.
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The DNA sequence capable of pairing with 5' CGATTAGT 3' is:
A. 5' CGATTAGT 3'. B. 5' GCTAATCA 3'. C. 3' CGATTAGT 5'. D. 3' GCTAATCA 5'. Clarify Question · What is the key concept addressed by the question? · What type of thinking is required? · What key words does the question contain and what do they mean? Gather Content · What do you know about base pairing? How does it relate to the question? Consider Possibilities · What other information is related to the question? Which information is most useful? Choose Answer · Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer? Reflect on Process · Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?
Which of the following is not a role for methylation?
A. Methylation can protect bacterial RNA from restriction enzymes that are used to destroy viral DNA. B. Methylation of DNA can act as an inhibitor of gene expression. C. Methylation of DNA can enhance binding of certain proteins. D. Methylation is important in directing mismatch repair to repair the correct DNA strand. E. Methylation in eukaryotes can affect the formation of Z-DNA.
In humans, which cell does NOT have a nucleus when mature?
a. erythrocytes b. lymphocytes c. neutrophils d. eosinophils e. monocytes
A typical cell cycle consists of
a. mitosis and meiosis. b. G1, the S phase, and G2. c. prophase, metaphase, anaphase, and telophase. d. interphase, mitosis, and cytokinesis. e. meiosis and fertilization.