Which of the following do I need to amplify the DNA for gene X and not for gene Y?

a. Some prior knowledge of the sequence of gene X so appropriate primers can be designed.
b. a heat-stable DNA polymerase
c. restriction endonucleases that cut gene X and not gene Y
d. plasmid vectors that have multiple antibiotic resistance genes.
e. A cDNA library and access to probes for gene X


A

Biology & Microbiology

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A solution contains 0.0000001 (10-7) moles of hydroxyl ions [OH-] per liter. Which of the following best describes this solution?

A) acidic: H+ acceptor B) basic: H+ acceptor C) acidic: H+ donor D) basic: H+ donor E) neutral

Biology & Microbiology

Embryonic cell cycles allow the rapid division of cells in the early embryo.  These mitotic cell cycles are much shorter in length than the mitotic cell cycles of cells in a mature organism.  In the embryonic cell cycles, mitosis takes approximately the same amount of time as it does in the cell cycles of mature cells.  What do you think is a result of the embryonic cycle?  

A.  Resulting daughter cells are smaller than the mother cell in the embryonic cell cycles. B.  Resulting daughter cells do not contain the same genetic information as the mother cell in the embryonic cell cycles. C.  Resulting daughter cells cannot form a mitotic spindle in the embryonic cell cycle. D.  Mother cells in the embryonic cell cycle spend the majority of their time in G0. Clarify Question · What is the key concept addressed by the question? · What type of thinking is required? · What key words does the question contain and what do they mean?   Gather Content   · What do you know about the cell cycle? How does it relate to the question?   Consider Possibilities     · What other information is related to the question? Which information is most useful?   Choose Answer   · Given what you now know, what information and/or problem solving approach is most likely to produce the correct answer?   Reflect on Process   · Did your problem-solving process lead you to the correct answer? If not, where did the process break down or lead you astray? How can you revise your approach to produce a more desirable result?

Biology & Microbiology

How would ecological efficiency change if all humans became vegetarians?

What will be an ideal response?

Biology & Microbiology

Which of the following mechanisms best describes the manner in which lysozyme lowers the energy required for its substrate to reach its transition-state conformation?

(a) by binding two molecules and orienting them in a way that favors a reaction between them (b) by altering the shape of the substrate to mimic the conformation of the transition state (c) by speeding up the rate at which water molecules collide with the substrate (d) by binding irreversibly to the substrate so that it cannot dissociate

Biology & Microbiology