Describe the three main components to PCR-based testing
What will be an ideal response?
Answer: The first step requires that nucleic acids be extracted from the sample, for example through bead-beating cell lysis. Amplification of the DNA or RNA is then performed using primers specifically targeting the nucleic acid sequence(s) of interest. Finally, observation of the PCR product can be done either with or without enhanced visualization systems, which generally first required the PCR amplicons to be electrophoresed.
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Which of these is a type of chemical bond that involves the sharing of electrons
a. hydrogen b. buffer c. surface tension d. ionic e. covalent
The optimal temperature and length of time to incubate a sample suspected of containing a dermatophyte is _____°C for _____ days
a. 25; 10 b. 25; 14 c. 30; 7 d. 30; 21
In order to reuse an enzyme after the conclusion of an enzyme catalyzed reaction, what must occur?
A. the enzyme has to be resynthesized B. the enzyme has to separate itself from the product C. the enzyme has to decrease entropy D. changes into an active form
Fragments of (LPS/NAM/NAG) released from Gram-negative bacteria into the bloodstream produce fever and shock
What will be an ideal response?