Describe the use of the DNA-degrading enzyme DNase I in experiments that explore the effect of chromatin on gene inactivation

What will be an ideal response?


DNA from cells with certain transcriptionally active vs. inactive genes can be isolated and treated with DNase I; then the condition of the genes are assessed with a Southern blot. Inactive, sequestered genes should remain intact, while active, exposed genes should have been degraded by the enzyme.

Biology & Microbiology

You might also like to view...

Define aneuploidy and describe the sex chromosome aneuploidies involved in Turner syndrome, Klinefelter syndrome, and XYY syndrome; list characteristics of each disorder

What will be an ideal response?

Biology & Microbiology

In what stage of a pregnancy are drugs used to

induce an abortion?

a. the first nine weeks b. the first twelve weeks c. the first 24 weeks d. up until the 30th week e. throughout the entire pregnancy

Biology & Microbiology

During aerobic cellular respiration, a proton gradient in mitochondria is generated by ________ and used primarily for ________.

A) the electron transport chain; ATP synthesis B) the electron transport chain; substrate-level phosphorylation C) glycolysis; production of H2O D) fermentation; NAD+ reduction E) diffusion of protons; ATP synthesis

Biology & Microbiology

Often one is not acclimated to the cold after having moved from a warm to cooler climate. Similarly, individuals may have difficulty adjusting to the heat after having moved from a cold to warm region. This difficulty in acclimating to different temperatures most likely requires adjustments in thyroid gland activity.

Answer the following statement true (T) or false (F)

Biology & Microbiology