You are working in a cancer lab and are interested in identifying some genes that are important in the onset of lung cancer. You have several genes you think may be involved, so you "print" spots of the DNA sequences of interest on a microarray slide. After doing this, you isolate mRNA from both normal lung cells and cancerous lung cells. Before you use these mRNAs to probe the microarray slide, you need to
A. sequence the mRNA.
B. make cDNA and amplify the cDNA using PCR.
C. clone each mRNA into a BAC vector.
D. amplify the mRNA using PCR.
E. add reverse transcriptase and fluorescent nucleotides to the mRNA to make cDNA.
Answer: E
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Potable water
A. is not suitable for human consumption. B. requires boiling to make it safe to drink. C. is suitable for human consumption. D. requires chemical or biological treatment to remove heavy metals.
Which technique did Rosalind Franklin use to determine many of the physical characteristics of DNA?
a. scanning electron microscopy b. transmission electron microscopy c. recombinant DNA techniques
d. x-ray crystallography e. DNA profiling
When tryptophan levels in a cell are low, a cell will stall transcription but continue translation of the tryptophan operon.
Answer the following statement true (T) or false (F)
Insects and humans are both categorized as animals. Describe how insects' respiratory system, circulatory system, and digestive system differ from humans.
What will be an ideal response?