Optimal conditions for the recovery of M. haemophilum from specimens include:

a. inoculation to a sheep blood agar plate.
b. incubation at 35°C to 42°C.
c. placement of an X-factor disk on the me-dia.
d. microaerophilic environment with low humidity.


C
A chocolate agar plate or the placement of an X-factor (hemin) disk on conventional media and incubation at 25°C to 33°C are needed for the recovery of M. haemophilum.

Biology & Microbiology

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