What is the Southern blotting method of DNA analysis? Who developed it?

What will be an ideal response?


A transfer method developed by Edward Southern, called Southern blotting, is used. In this process, the DNA is transferred to a nylon membrane in much the same way that ink is transferred to a blotter. The nylon sheet is then treated with radioactively labeled DNA probes, single-stranded pieces of DNA that can bind through complementary base pairing with target DNA. Whereas a single-locus probe "looks" for only one field, a multilocus probe looks for numerous fields simultaneously. The radioactive probe then merges with the specific DNA sequences found on the membrane fragments. The images that result from X-ray film placed in contact with the membrane to detect the probe configuration look like the price bar codes used on supermarket products.

Criminal Justice

You might also like to view...

Police applicants must successfully complete the ________ process before being hired

Fill in the blank(s) with correct word

Criminal Justice

Subject-matter jurisdiction refers to the power of the Court to determine whether a law or action by the other branches of the government is constitutional.

Answer the following statement true (T) or false (F)

Criminal Justice

The ______ carries out the basic housekeeping functions of the body by funneling messages from the environment to the various internal organs so that they may keep the organism in a state of biological balance.

a. ANS b. EEG c. PCL-R d. RAS

Criminal Justice

Why is digital piracy a problem in America?

What will be an ideal response?

Criminal Justice