Proteins used to digest DNA at specific sequence regions are:
a) Protein Kinases
b) Phospholipases
c) Restriction Enzymes
d) RNA Replicases
Answer: c) Restriction Enzymes
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Which situation(s) might warrant the removal of a histidine tag when purifying a recombinant protein?
A. Histidine residues inhibit protein folding, thereby decreasing the functionality of the protein. B. The expression vector used for inserting the gene of interest already contains histidine-encoding sequences. C. Histidine residues bind preferentially to nickel resins on the column during purification, and are not washed away. D. All of the choices are correct.
The gene that encodes small-subunit ribosomal RNA is useful for reconstructing the phylogeny of all organisms, because ________
A) the gene is subject to strong stabilizing selection B) the gene's function has stayed unchanged in all organisms C) all organisms have ribosomes with similar composition and structure D) all organisms have the gene E) All of the above.
The critical hurdle with measuring treatment effects is that:
A. our subjects cannot be both untreated and treated at the same time. B. it's always equal to the effect of the treatment on the treated. C. it's hard to have a truly random treatment assignment. D. we have selection bias.
The templates for next-generation sequencing are
A) double-strand restriction fragments. B) double-strand DNA on silicon chips. C) double-strand PCR products. D) single-strand DNA on silicon chips. E) single-strand DNA on slides.