After being stained with crystal violet, how will a gram-positive gonococcus react?
a. Fluoresce after counterstain is applied.
b. Accept the counterstain.
c. Retain the original stain after the coun-terstain is applied.
d. Turn dark after the counterstain is applied.
C
Staining procedures differentiate organisms by using dyes that have been found to stain some bacteria in specific ways. An example of this would be a Gram stain, in which bacteria are first stained with crystal violet, then treated with a strong iodine solution, decolorized with ethanol or ethanol acetone, and then counterstained with contrasting dye. Those retaining the initial stain are considered gram positive; those losing the stain but accepting the counterstain are considered gram negative.
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