When cloning DNA into bacteria,
a. the DNA sequence is inserted into a plasmid.
b. the DNA sequence is inserted into the bacterial chromosome.
c. the linear DNA sequence is circularized, thus generating a plasmid.
d. the new DNA is directly introduced into the bacterial cell.
e. either the approach in b or c is used; the choice depends on the type of bacteria.
A
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Hydrolysis of sucrose, a disaccharide, results in
A. two nucleotides. B. two monomers. C. two glycerol units. D. the release of functional groups. E. three monosaccharides.
In TILLING, a population of inbred organisms is mutagenized to saturation. DNA from mutagenized lines is isolated and amplified by PCR. PCR products are denatured and allowed to reanneal and then treated with an endonuclease
Why is the denaturation/re-annealing step important for the detection of mutant alleles?
Regular refrigeration does not inhibit the growth of ________ microbes that can continue to grow and cause food spoilage.
What will be an ideal response?
You are given a new animal species to study. You note that it possesses segmentation. Based entirely on the knowledge that it is segmented, to which of these phyla might the new species belong? Check all that apply.
A. Chordata. B. Arthropoda. C. Cnidaria. D. Platyhelminthes. E. Annelida.