You are studying biology with your classmate when he asks what the difference is between Sanger sequencing and PCR with regard to the materials needed to perform these reactions. You tell him:
A. In PCR, primers are needed but not in
Sanger sequencing.
B. In PCR, DNA polymerase is needed but
not in Sanger sequencing.
C. In Sanger sequencing, primers are needed
but not in PCR.
D. In Sanger sequencing, DNA polymerase is
needed but not in PCR.
E. In Sanger sequencing, dideoxynucleotides
are needed, but not in PCR.
E
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Microarrays are created by robotically placing DNA on to a microscope slide and probing with
A. RNA from the tissue of interest. B. another DNA from the tissue of interest. C. SNPs from the tissue of interest. D. STSs from the tissue of interest. E. clone-by-clone sequencing.
If ATP production was regulated by positive feedback, then high levels of ATP in muscle cells would
A. stimulate more ATP production. B. inhibit more ATP production. C. not alter ATP production. D. stimulate ATP degradation. E. All of the answers for this question are correct.
If bacteria are infected with a bacteriophage and briefly exposed to radioactive uracil, what results would you expect to see immediately after exposure?
A) radioactive mRNA in the cytoplasm B) radioactive mRNA in the nucleus C) radioactive DNA in the cytoplasm D) radioactive DNA in the nucleus E) radioactive proteins in the nuclear membrane
If you had the ability to genetically engineer a pathogenic bacterium, what features would you include? Choose a portal of entry and a portal of exit for your pathogen. Then choose three virulence factors from at least two the following areas: toxins,
adhesins, invasins, acquisition of nutrients, and avoidance of the immune system, and describe how each factor will help your bacterium survive in the host. Your virulence factors should make sense for the portals of entry and exit that you chose. What will be an ideal response?