Assume that the gene trpA in an auxotrophic strain of E. coli is located at 27 minutes, whereas the gene pyrE is located at 81 minutes. How are minutes arrived at in the context of gene mapping in bacteria?
What will be an ideal response?
(a) Minutes are arrived at by an Hfr X F- mating as in the Klug and Cummings text.
(b) Hfr (wild type) X F- (auxotroph)
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During the tube gel electrophoresis phase of 2-D gel electrophoresis, proteins are separated based on their net charge at a given pH. Each protein will migrate to a point in the gel where its net charge is zero. This process is known as what?
A. isoelectric focusing B. electrophoretic honing C. migratory centering D. hypoelectric centering E. hyperelectric focusing
Bacteriorhodopsin is a light-sensitive pigment found in ____ archaeans used to supply energy for ____ production
a. halophilic; ATP b. thermophilic; carbohydrate c. methanogenic; methane d. halophilic; carbohydrate e. thermophilic; ATP
Food that is produced using unsustainable farming practices tends to
A) be less susceptible to pest damage. B) have a lower fat content. C) have more complex carbohydrates. D) have a longer shelf life. E) be cheaper.
The process whereby a cell's eventual developmental fate is set is called ________
A) notching B) embryogenisis C) differentiation D) determination