What is the sequence of the DNA synthesized in the sequencing reaction?
You performed a Sanger sequencing reaction and obtained the following read. In this figure, A = green, C = purple, G = black, T = red. The height of the peaks is unimportant. The smallest molecule is at the left of the trace.
A) 5' TTTGCTTTGTGAGCGGATAACAA 3'
B) 3' TTTGCTTTGTGAGCGGATAACAA 5'
C) 5' AAACGAAACACTCGCCTATTGTT 3'
D) 3' AAACGAAACACTCGCCTATTGTT 5'
A) 5' TTTGCTTTGTGAGCGGATAACAA 3'
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